FWS Midwest Region 3 FAC Bighead and Silver Carp eDNA Data Feature Layer View [U.S. Fish and Wildlife Service] Full Details
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Full Details
- Title:
- FWS Midwest Region 3 FAC Bighead and Silver Carp eDNA Data Feature Layer View [U.S. Fish and Wildlife Service]
- Description:
- The Invasive Carp environmental DNA (eDNA) sample data was collected and processed by the U.S. Fish and Wildlife Service (USFWS) and is used for the early detection and monitoring of Invasive carp. eDNA detection summary data along with static maps are shared with the public. For further information on data collection and processing please refer to the Quality Assurance Project Plan eDNA Monitoring of Bighead and Silver Carps. Additional information on the Invasive carp eDNA program for the U.S. Fish and Wildlife Service along with all result eDNA data can be found on the main web page (coming soon). A positive eDNA detection result means there was Invasive carp eDNA in the water body, which can be from live or dead fish, but it could have also been transported via boat, bird, or water current. A positive eDNA detection does not necessarily mean there were Invasive carp present at the time samples were taken. For more information or questions, please contact the eDNA Program Coordinator, Nick Frohnauer at nicholas_frohnauer@fws.govThumbnail Photo Credit: Gretchen Newberry, USFWS
- Creator:
- Department of the Interior
- Provider:
- U.S. Fish and Wildlife Service Open Data
- Resource Class:
- Web services
- Temporal Coverage:
- Last modified 2021-10-05
- Date Issued:
- 2021-05-04
- Place:
- Rights:
- The United States Fish and Wildlife Service (Service) shall not be held liable for improper or incorrect use of the data described and/or contained herein. While the Service makes every reasonable effort to ensure the accuracy and completeness of data provided for distribution, it may not have the necessary accuracy or completeness required for every possible intended use. The Service recommends that data users consult the associated metadata record to understand the quality and possible limitations of the data. The Service creates metadata records in accordance with the standards endorsed by the Federal Geographic Data Committee.As a result of the above considerations, the Service gives no warranty, expressed or implied, as to the accuracy, reliability, or completeness of the data. It is the responsibility of the data user to use the data in a manner consistent with the limitations of geospatial data in general and these data in particular. Although these data have been processed successfully on a computer system at the Service, no warranty, expressed or implied, is made regarding the utility of the data on another system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. This applies to the use of the data both along and in aggregate with other data and information.Please pay special attention to the feature layer metadata and to the Quality Assurance Project Plan for the eDNA Monitoring of Bighead and Silver Carp.Data Interpretation ConsiderationsA blank sample is a field control sample comprised of a sample tube filled with distilled water prior to field collection. Blanks are not true monitoring samples but are instead a quality control measure used to ensure that contamination is not occurring between samples. A positive eDNA detection result means there was Invasive carp DNA in the water body, which can be from live fish, dead fish, or a secondary vector. Secondary vectors are things like barges, boats, birds, and sewer systems that may potentially transport Invasive carp DNA to the area of detection from somewhere else. A positive eDNA detection does not necessarily mean there were Invasive carp (Bighead and/or Silver carp) present at the time samples were taken. eDNA collection density and strategy in the field has evolved from 2013 to present and has ranged from collecting transects across large areas to targeted, high-density sampling in areas where eDNA can accumulate. Differences in sample collection technique and distribution should be considered when comparing eDNA data across years. The genetic markers used to detect Invasive carp eDNA changed in 2014. In 2015, the detection methods used changed from conventional PCR (cPCR) to quantitative PCR (qPCR). Due to these changes, eDNA detection data prior to 2015 are not the same as eDNA detection data from 2015-present. The process used to concentrate the eDNA from a water sample has changed over time; from filtering samples (2013-2014) to centrifugation (2015-present). Caution should be taken when comparing detections from the two methods together. In 2020, the genetic markers used to detect Invasive carp eDNA changed from single marker qPCR to a multi-marker (multiplex) qPCR reaction, increasing efficiency and consistency. Decontamination of all boats, personal equipment, and nets is standard practice due to the Environmental DNA Calibration study (URL). eDNA detections prior to 2014 should be carefully interpreted because it is very likely that the majority, if not all, of the detections from that time period could have resulted from unintentional cross-contamination or movement of genetic material from downstream carp populations to the CAWs in the absence of live Invasive carp.
- Access Rights:
- Public
- Format:
- File
- Language:
- English
- Date Added:
- 2023-08-11